Research Article
The effects of exercise intensity on the gut microbiota of college basketball players
Hong-xian Deng, Huan-huan Li, Jiu-quan Qiao*, Yan Tong*, Cui-juan Wang and Jiang Liu
Published: 04 March, 2023 | Volume 4 - Issue 1 | Pages: 010-018
Purpose: Exercise has a positive regulatory effect on gut microbiota and is also involved in regulating multiple physiological functions of the human body. This article tested the effects of basketball exercises at different exercise intensities on the gut microbiota of college students.
Methods: Athlete research subjects (male, aged 18 - 25) were selected from the basketball team and trained at different intensities to obtain a total of 101 fresh fecal samples. DNA was extracted by a DNA extraction kit and bacterial 16S rRNA gene V3-V4 region high-throughput sequencing using the Illumina Hiseq platform. The downstream data were spliced, filtered and de-trimerized and then used to study the difference in gut microbiota.
Results: Key bacterial taxa in the gut that responded to exercise intensity differed among athletes of different exercise intensities but most belonged to Firmicutes. With increasing exercise intensity, Butyricicoccus, Anaerostipes, Oxalobacter and Clostridium_IV in basketball players enrich. Further analysis of the functional prediction revealed that carbohydrate metabolism, amino acid metabolism, metabolism of xenobiotics and glycans and metabolism were significantly expressed in the gut microbiota of basketball athletes with high intensity.
Conclusion: The study demonstrated that after long-term professional training, the gut microbiota of athletes adapts to exercise stimulators and can quickly respond to changes in exercise intensity. In high-intensity training, the organism is protected from harm by enriching some beneficial bacteria.
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DOI: 10.29328/journal.jcmhs.1001028
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Keywords:
Athletes; Exercise intensity; Gut microbiota; High-throughput sequencing
References
- Silverman MN, Deuster PA. Biological mechanisms underlying the role of physical fitness in health and resilience. Interface Focus. 2014 Oct 6;4(5):20140040. doi: 10.1098/rsfs.2014.0040. PMID: 25285199; PMCID: PMC4142018.
- Mitchell CM, Davy BM, Hulver MW, Neilson AP, Bennett BJ, Davy KP. Does Exercise Alter Gut Microbial Composition? A Systematic Review. Med Sci Sports Exerc. 2019 Jan;51(1):160-167. doi: 10.1249/MSS.0000000000001760. PMID: 30157109.
- Kudelka MR, Stowell SR, Cummings RD, Neish AS. Intestinal epithelial glycosylation in homeostasis and gut microbiota interactions in IBD. Nat Rev Gastroenterol Hepatol. 2020 Oct;17(10):597-617. doi: 10.1038/s41575-020-0331-7. Epub 2020 Jul 24. PMID: 32710014; PMCID: PMC8211394.
- Bressa C, Bailén-Andrino M, Pérez-Santiago J, González-Soltero R, Pérez M, Montalvo-Lominchar MG, Maté-Muñoz JL, Domínguez R, Moreno D, Larrosa M. Differences in gut microbiota profile between women with active lifestyle and sedentary women. PLoS One. 2017 Feb 10;12(2):e0171352. doi: 10.1371/journal.pone.0171352. PMID: 28187199; PMCID: PMC5302835.
- Mohr AE, Jäger R, Carpenter KC, Kerksick CM, Purpura M, Townsend JR, West NP, Black K, Gleeson M, Pyne DB, Wells SD, Arent SM, Kreider RB, Campbell BI, Bannock L, Scheiman J, Wissent CJ, Pane M, Kalman DS, Pugh JN, Ortega-Santos CP, Ter Haar JA, Arciero PJ, Antonio J. The athletic gut microbiota. J Int Soc Sports Nutr. 2020 May 12;17(1):24. doi: 10.1186/s12970-020-00353-w. PMID: 32398103; PMCID: PMC7218537.
- Donati Zeppa S, Agostini D, Gervasi M, Annibalini G, Amatori S, Ferrini F, Sisti D, Piccoli G, Barbieri E, Sestili P, Stocchi V. Mutual Interactions among Exercise, Sport Supplements and Microbiota. Nutrients. 2019 Dec 20;12(1):17. doi: 10.3390/nu12010017. PMID: 31861755; PMCID: PMC7019274.
- Keohane DM, Woods T, O'Connor P, Underwood S, Cronin O, Whiston R, O'Sullivan O, Cotter P, Shanahan F, Molloy MGM. Four men in a boat: Ultra-endurance exercise alters the gut microbiome. J Sci Med Sport. 2019 Sep;22(9):1059-1064. doi: 10.1016/j.jsams.2019.04.004. Epub 2019 Apr 18. PMID: 31053425.
- Lynch SV, Pedersen O. The Human Intestinal Microbiome in Health and Disease. N Engl J Med. 2016 Dec 15;375(24):2369-2379. doi: 10.1056/NEJMra1600266. PMID: 27974040.
- Belcher BR, Zink J, Azad A, Campbell CE, Chakravartti SP, Herting MM. The Roles of Physical Activity, Exercise, and Fitness in Promoting Resilience During Adolescence: Effects on Mental Well-Being and Brain Development. Biol Psychiatry Cogn Neurosci Neuroimaging. 2021 Feb;6(2):225-237. doi: 10.1016/j.bpsc.2020.08.005. Epub 2020 Aug 18. PMID: 33067166; PMCID: PMC7878276.
- Clarke SF, Murphy EF, O'Sullivan O, Lucey AJ, Humphreys M, Hogan A, Hayes P, O'Reilly M, Jeffery IB, Wood-Martin R, Kerins DM, Quigley E, Ross RP, O'Toole PW, Molloy MG, Falvey E, Shanahan F, Cotter PD. Exercise and associated dietary extremes impact on gut microbial diversity. Gut. 2014 Dec;63(12):1913-20. doi: 10.1136/gutjnl-2013-306541. Epub 2014 Jun 9. PMID: 25021423.
- Codella R, Luzi L, Terruzzi I. Exercise has the guts: How physical activity may positively modulate gut microbiota in chronic and immune-based diseases. Dig Liver Dis. 2018 Apr;50(4):331-341. doi: 10.1016/j.dld.2017.11.016. Epub 2017 Nov 28. PMID: 29233686.
- Fugmann M, Breier M, Rottenkolber M, Banning F, Ferrari U, Sacco V, Grallert H, Parhofer KG, Seissler J, Clavel T, Lechner A. The stool microbiota of insulin resistant women with recent gestational diabetes, a high risk group for type 2 diabetes. Sci Rep. 2015 Aug 17;5:13212. doi: 10.1038/srep13212. PMID: 26279179; PMCID: PMC4538691.
- Mejía-León ME, Petrosino JF, Ajami NJ, Domínguez-Bello MG, de la Barca AM. Fecal microbiota imbalance in Mexican children with type 1 diabetes. Sci Rep. 2014 Jan 22;4:3814. doi: 10.1038/srep03814. PMID: 24448554; PMCID: PMC3898044.
- Li C, Huang Q, Yang R, Dai Y, Zeng Y, Tao L, Li X, Zeng J, Wang Q. Gut microbiota composition and bone mineral loss-epidemiologic evidence from individuals in Wuhan, China. Osteoporos Int. 2019 May;30(5):1003-1013. doi: 10.1007/s00198-019-04855-5. Epub 2019 Jan 21. PMID: 30666372.
- Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C. Metagenomic biomarker discovery and explanation. Genome Biol. 2011 Jun 24;12(6):R60. doi: 10.1186/gb-2011-12-6-r60. PMID: 21702898; PMCID: PMC3218848.
- Rawson RA, Chudzynski J, Gonzales R, Mooney L, Dickerson D, Ang A, Dolezal B, Cooper CB. The Impact of Exercise On Depression and Anxiety Symptoms Among Abstinent Methamphetamine-Dependent Individuals in A Residential Treatment Setting. J Subst Abuse Treat. 2015 Oct;57:36-40. doi: 10.1016/j.jsat.2015.04.007. Epub 2015 Apr 15. PMID: 25934458; PMCID: PMC4560957.
- Barton W, Penney NC, Cronin O, Garcia-Perez I, Molloy MG, Holmes E, Shanahan F, Cotter PD, O'Sullivan O. The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level. Gut. 2018 Apr;67(4):625-633. doi: 10.1136/gutjnl-2016-313627. Epub 2017 Mar 30. PMID: 28360096.
- Dziewiecka H, Buttar HS, Kasperska A, Ostapiuk-Karolczuk J, Domagalska M, Cichoń J, Skarpańska-Stejnborn A. Physical activity induced alterations of gut microbiota in humans: a systematic review. BMC Sports Sci Med Rehabil. 2022 Jul 7;14(1):122. doi: 10.1186/s13102-022-00513-2. PMID: 35799284; PMCID: PMC9264679.
- Ramos C, Gibson GR, Walton GE, Magistro D, Kinnear W, Hunter K. Systematic Review of the Effects of Exercise and Physical Activity on the Gut Microbiome of Older Adults. Nutrients. 2022 Feb 5;14(3):674. doi: 10.3390/nu14030674. PMID: 35277033; PMCID: PMC8837975.
- Shanahan F. Probiotics in perspective. Gastroenterology. 2010 Dec;139(6):1808-12. doi: 10.1053/j.gastro.2010.10.025. Epub 2010 Oct 19. PMID: 20965190.
- Cani PD, Amar J, Iglesias MA, Poggi M, Knauf C, Bastelica D, Neyrinck AM, Fava F, Tuohy KM, Chabo C, Waget A, Delmée E, Cousin B, Sulpice T, Chamontin B, Ferrières J, Tanti JF, Gibson GR, Casteilla L, Delzenne NM, Alessi MC, Burcelin R. Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes. 2007 Jul;56(7):1761-72. doi: 10.2337/db06-1491. Epub 2007 Apr 24. PMID: 17456850.
- Le Chatelier E, Nielsen T, Qin J, Prifti E, Hildebrand F, Falony G, Almeida M, Arumugam M, Batto JM, Kennedy S, Leonard P, Li J, Burgdorf K, Grarup N, Jørgensen T, Brandslund I, Nielsen HB, Juncker AS, Bertalan M, Levenez F, Pons N, Rasmussen S, Sunagawa S, Tap J, Tims S, Zoetendal EG, Brunak S, Clément K, Doré J, Kleerebezem M, Kristiansen K, Renault P, Sicheritz-Ponten T, de Vos WM, Zucker JD, Raes J, Hansen T; MetaHIT consortium; Bork P, Wang J, Ehrlich SD, Pedersen O. Richness of human gut microbiome correlates with metabolic markers. Nature. 2013 Aug 29;500(7464):541-6. doi: 10.1038/nature12506. PMID: 23985870.
- Matsuoka K, Kanai T. The gut microbiota and inflammatory bowel disease. Semin Immunopathol. 2015 Jan;37(1):47-55. doi: 10.1007/s00281-014-0454-4. Epub 2014 Nov 25. PMID: 25420450; PMCID: PMC4281375.
- Eslami M, Yousefi B, Kokhaei P, Hemati M, Nejad ZR, Arabkari V, Namdar A. Importance of probiotics in the prevention and treatment of colorectal cancer. J Cell Physiol. 2019 Aug;234(10):17127-17143. doi: 10.1002/jcp.28473. Epub 2019 Mar 25. PMID: 30912128.
- Chang SC, Shen MH, Liu CY, Pu CM, Hu JM, Huang CJ. A gut butyrate-producing bacterium Butyricicoccus pullicaecorum regulates short-chain fatty acid transporter and receptor to reduce the progression of 1,2-dimethylhydrazine-associated colorectal cancer. Oncol Lett. 2020 Dec;20(6):327. doi: 10.3892/ol.2020.12190. Epub 2020 Oct 5. PMID: 33101496; PMCID: PMC7577080.
- Gui Q, Li H, Wang A, Zhao X, Tan Z, Chen L, Xu K, Xiao C. The association between gut butyrate-producing bacteria and non-small-cell lung cancer. J Clin Lab Anal. 2020 Aug;34(8):e23318. doi: 10.1002/jcla.23318. Epub 2020 Mar 29. PMID: 32227387; PMCID: PMC7439349.
- Wang Y, Huang D, Chen KY, Cui M, Wang W, Huang X, Awadellah A, Li Q, Friedman A, Xin WW, Di Martino L, Cominelli F, Miron A, Chan R, Fox JG, Xu Y, Shen X, Kalady MF, Markowitz S, Maillard I, Lowe JB, Xin W, Zhou L. Fucosylation Deficiency in Mice Leads to Colitis and Adenocarcinoma. Gastroenterology. 2017 Jan;152(1):193-205.e10. doi: 10.1053/j.gastro.2016.09.004. Epub 2016 Sep 14. PMID: 27639802; PMCID: PMC5164974.
- Chamberlain CA, Hatch M, Garrett TJ. Metabolomic and lipidomic characterization of Oxalobacter formigenes strains HC1 and OxWR by UHPLC-HRMS. Anal Bioanal Chem. 2019 Jul;411(19):4807-4818. doi: 10.1007/s00216-019-01639-y. Epub 2019 Feb 11. PMID: 30740635; PMCID: PMC6612311.
- Hoppe B, Leumann E, Milliner DS. CHAPTER 33 - Urolithiasis and Nephrocalcinosis in Childhood. In Comprehensive Pediatric Nephrology eds. Geary DF, Schaefer. Philadelphia: Mosby. 2008; 499-525.
- Guo P, Zhang K, Ma X, He P. Clostridium species as probiotics: potentials and challenges. J Anim Sci Biotechnol. 2020 Feb 20;11:24. doi: 10.1186/s40104-019-0402-1. PMID: 32099648; PMCID: PMC7031906.
- Atarashi K, Tanoue T, Oshima K, Suda W, Nagano Y, Nishikawa H, Fukuda S, Saito T, Narushima S, Hase K, Kim S, Fritz JV, Wilmes P, Ueha S, Matsushima K, Ohno H, Olle B, Sakaguchi S, Taniguchi T, Morita H, Hattori M, Honda K. Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota. Nature. 2013 Aug 8;500(7461):232-6. doi: 10.1038/nature12331. Epub 2013 Jul 10. PMID: 23842501.
- Rivière A, Selak M, Lantin D, Leroy F, De Vuyst L. Bifidobacteria and Butyrate-Producing Colon Bacteria: Importance and Strategies for Their Stimulation in the Human Gut. Front Microbiol. 2016 Jun 28;7:979. doi: 10.3389/fmicb.2016.00979. PMID: 27446020; PMCID: PMC4923077.
- Wang SZ, Yu YJ, Adeli K. Role of Gut Microbiota in Neuroendocrine Regulation of Carbohydrate and Lipid Metabolism via the Microbiota-Gut-Brain-Liver Axis. Microorganisms. 2020 Apr 7;8(4):527. doi: 10.3390/microorganisms8040527. PMID: 32272588; PMCID: PMC7232453.
- Lyu Z, Wang L, Wang J, Wang Z, Zhang S, Wang J, Cheng J, Lai C. Oat bran and wheat bran impact net energy by shaping microbial communities and fermentation products in pigs fed diets with or without xylanase. J Anim Sci Biotechnol. 2020 Oct 8;11:99. doi: 10.1186/s40104-020-00505-7. PMID: 33062263; PMCID: PMC7542896.
- Reynolds LA, Finlay BB. A case for antibiotic perturbation of the microbiota leading to allergy development. Expert Rev Clin Immunol. 2013 Nov;9(11):1019-30. doi: 10.1586/1744666X.2013.851603. PMID: 24168410.
- Bai Y, Mansell TJ. Production and Sensing of Butyrate in a Probiotic Escherichia coli Int J Mol Sci. 2020 May 20;21(10):3615. doi: 10.3390/ijms21103615. PMID: 32443851; PMCID: PMC7279287.
- Artioli GG, Gualano B, Smith A, Stout J, Lancha AH Jr. Role of beta-alanine supplementation on muscle carnosine and exercise performance. Med Sci Sports Exerc. 2010 Jun;42(6):1162-73. doi: 10.1249/MSS.0b013e3181c74e38. PMID: 20479615.
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